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101.
The long preservation and recovery of functional (contractile) properties in cultured aortic smooth muscle cells, even after replating or deep-frozen storage and the measurement of their responses are now technically settled issues. We could thus study extensively the responses of single cultured cells from rat thoracic aorta. Responses were elicited by the addition of KCl 40 mmol/L without or with a calcium blocker PN 200-100 (10–6 mol/L); angiostein II (10–11–10–6 mol/L) without or with antagonist (losartan 10–5 mol/L); or serotonin (10–9–10–4 mol/L) without or with antagonist (naftidrofuryl 10–5 mol/L). Results thus obtained enabled us to propose a new hypothesis for the interpretation of the contractile responses of an elastic vascular smooth muscle. The different maximal effects of different agonists result mainly from the different proportions of cells they can mobilize; the agonist concentration-contraction relationship is mainly due to the increase of the proportion of cells involved up to a maximal value typical of the agonist used. An antagonist primarily reduce the proportion of cells an agonist can mobilize. Some of the consequences of this hypothesis are briefly outlined. 相似文献
102.
Forest age structure is one of the main indicators of biodiversity in temperate and boreal forests worldwide. This indicator was mainly chosen for the conservation of a subset of rare or sensitive species related to the oldest age classes, not to capture variability across the entire biodiversity spectrum, but is often considered as such. In this study, we analysed alpha and beta diversity in temporary plots of western Quebec, Canada, to consider biodiversity indicators complementary to existing forest age structure targets. Our analysis revealed that considered individually, stand characteristics such as cover type and height are better predictors of changes in site-level contribution to tree beta diversity than age. We also show that plots belonging to different age classes can be similar in terms of tree alpha diversity. Height class was found to have a more significant impact on tree alpha diversity than expected: height was more important than age in coniferous forests, and in deciduous and mixedwood stands it frequently complemented age in explaining the observed diversity patterns. Our results suggest that forest age structure target levels should not be used as the sole indicator of ecosystem sustainability, and that some mature secondary stands can provide significant contributions to biodiversity. We propose that more efficient trade-offs between forest exploitation, ecosystem functioning and environmental conservation can be attained if: (i) forest age structure targets are complemented by cover type and stand height; or (ii) complementary biodiversity indicators of ecosystem sustainability are implemented. 相似文献
103.
The genetic diversity among the Turkish cultivars of common bean (Phaseolus vulgaris L.) was estimated by studying the Sequence Related Amplified Polymorphism (SRAP), Peroxidase Gene Polymorhism (POGP), and Chloroplast Simple Sequence Repeats (cpSSR) markers. The unweighted pair group method arithmetic average (UPGMA) and Neighbor joining (NJ) algorithm resulted in a dendrogram representing the genetic relationship among major common bean cultivars grown in Turkey. The dendrogram generated two groups possibly representing two different major gene pools. By using three different marker systems, 194 alleles were detected and 118 were found to be polymorphic. For SRAP, POGP and cpSSR, 64, 64 and 26% polymorphism ratio were obtained, respectively. Principal Component Analysis (PCA) was also carried out to determine genetic variation among common bean genotypes and three different groups were generated. The individuals were placed into three different populations in structure analysis. Three populations created in structure analysis were exactly corresponded to the three groups in PCA. Analysis of Molecular Variance (AMOVA) was used to partition the genetic variations. The percentage of the variance was approximately 59%, 3%, and 38% among groups, among populations within groups and, within populations, respectively. The percentages of variation were found to be significantly high within the populations and among the groups. 相似文献
104.
Tropical cowpea rhizobia are often presumed to be generally promiscuous but poor N fixers. This study was conducted to evaluate symbiotic interactions of 59 indigenous rhizobia isolates (49 of them from cowpea (Vigna unguiculata)), with up to 13 other (mostly tropical) legume species. Host ranges averaged 2.4 and 2.3 legume species each for fast- and slow-growing isolates respectively compared to 4.3 for slow-growing reference cowpea strains. An average of 22% and 19% of fast- and slow-growing cowpea isolates respectively were effective on each of 12 legume species tested. We conclude that the indigenous cowpea rhizobia studied have relatively narrow host ranges. The ready nodulation of different legumes in tropical soils appears due to the diversity of indigenous symbiotic genotypes, each consisting of subgroups compatible with a limited number of legume species. 相似文献
105.
《Systematic and applied microbiology》2020,43(2):126053
Bradyrhizobium are N2-fixing microsymbionts of legumes with relevant applications in agricultural sustainability, and we investigated the phylogenetic relationships of conserved and symbiotic genes of 21 bradyrhizobial strains. The study included strains from Western Australia (WA), isolated from nodules of Glycine spp. the country is one genetic center for the genus and from nodules of other indigenous legumes grown in WA, and strains isolated from forage Glycine sp. grown in South Africa. The 16S rRNA phylogeny divided the strains in two superclades, of B. japonicum and B. elkanii, but with low discrimination among the species. The multilocus sequence analysis (MLSA) with four protein-coding housekeeping genes (dnaK, glnII, gyrB and recA) pointed out seven groups as putative new species, two within the B. japonicum, and five within the B. elkanii superclades. The remaining eleven strains showed higher similarity with six species, B. lupini, B. liaoningense, B. yuanmingense, B. subterraneum, B. brasilense and B. retamae. Phylogenetic analysis of the nodC symbiotic gene clustered 13 strains in three different symbiovars (sv. vignae, sv. genistearum and sv. retamae), while seven others might compose new symbiovars. The genetic profiles of the strains evaluated by BOX-PCR revealed high intra- and interspecific diversity. The results point out the high level of diversity still to be explored within the Bradyrhizobium genus, and further studies might confirm new species and symbiovars. 相似文献
106.
One of the greatest threats to biodiversity and the sustainable functioning of ecosystems is the clearing of forests for agriculture. Because litter-dwelling ants are very good bioindicators of man-made disturbance, we used them to compare monospecific plantations of acacia trees, cocoa trees, rubber trees and pine trees with the surrounding Neotropical rainforest (in contrast to previous studies on forest fragments embedded in industrial monocultures). Although the global level of species turnover was weak, species richness decreased along a gradient from the forest (including a treefall gap) to the tree plantations among which the highest number of species was noted for the cocoa trees, which are known to be a good compromise between agriculture and conservation. Species composition was significantly different between natural habitats and the plantations that, in turn, were different from each other. Compared to the forest, alterations in the ant communities were (1) highest for the acacia and rubber trees, (2) intermediate for the cocoa trees, and, (3) surprisingly, far lower for the pine trees, likely due to very abundant litter. Functional traits only separated the rubber tree plantation from the other habitats due to the higher presence of exotic and leaf-cutting ants. This study shows that small monospecific stands are likely sustainable when embedded in the rainforest and that environmentally-friendly strategies can be planned accordingly. 相似文献
107.
C. W. Cunningham 《Biological invasions》2008,10(1):1-6
The rapid range southward expansion of the periwinkle Littorina littorea from the Canadian maritimes has fueled a long-running debate over whether this species was introduced to North America by
human activity. A reappraisal of the mitochondrial DNA sequence evidence finds considerable endemic allelic diversity in the
American population. The degree of endemic genetic diversity is higher than expected from human-mediated colonization, but
not so much to suggest that it survived the last glacial maximum in America. Coalescent estimates of population divergence
agree that colonization of America preceded European contact. A reappraisal of the ITS nuclear sequence data finds extensive
recombination. Taking this recombination into account strengthens the genetic case against human-mediated introduction. Finally,
a reappraisal of conflicting allozyme studies from the 1970’s supports a claim of limited divergence between American and
European populations. This is consistent with post-glacial colonization, but the allozyme data cannot distinguish between
natural or human-mediated colonization. Taken as a whole, the DNA sequence data supports the many sub-fossil reports of an
American L. littorea population in the Canadian maritimes that preceded even the first visits by the Vikings. 相似文献
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